Transient transfection at large scale for clinical AAV9 vector manufacturing
AAV9 production in adherent HEK293 cells was scaled from the pilot iCELLis® Nano bioreactor to the large scale iCELLis 500 bioreactor (200 m2 and 333 m2 surface area). A HEK293 cell seed train was utilized, using the Xpansion® 200 bioreactor. Media components were evaluated and replenished as needed throughout the run using the perfusion process of the iCELLis bioreactor. Following a production phase, >1016 vector genomes were isolated from crude harvest of the bioreactor.
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Transient transfection at large scale for clinical AAV9 vector manufacturing
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July 2022
Jul
2022
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Successful Scale Up of a High Yielding Viral Vector Suspension Platform to 500 L
Biotech | Pall Corporation webinar series
Biotech | Pall Corporation webinar series
How Andelyn and Pall worked together to scale up an AAV manufacturing process to 500L