Screening clones to identify those carrying the desired genomic changes and expressing the target protein, can be carried out through traditional approaches such as: western blotting, enzyme-linked immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS), flow cytometry, or utilizing high content imaging systems. Maintaining sterility and clonality is important for purity and homogeneity of the target protein. Screening large numbers of clones as quickly as possible to select those of value for further analysis is essential.
In addition to sterile filtration systems to ensure media and environmental sterility, Pall Laboratory offers centrifugal devices and filter plates which simplify protein sample preparation, gDNA and mRNA purification. The AcroPrep™ Advance filter plate is designed to reduce the risk of cross-contamination and create a smooth liquid flow for sample processing and high-throughput screening. Our high-sensitivity protein binding membranes designed for protein detection analysis applications, such as western blotting, exhibit low background noise for clarity and sensitivity of results. Immobilized proteins can be used directly for sequencing, providing flexibility in the workflow.
Ensure clear results for flow cytometry and cell labeling by preparing samples with AcroPrep Advance filter plates equipped with 30-40 µm PP/PE media. These plates are designed to capture clumps of cellular aggregates that could otherwise clog the flow chamber, disrupt the workflow, and impact clarity of results. All cell labeling and detection processes can be carried out in one plate (detection of cell surface antigens or intracellular proteins) without the need for aspiration after centrifugation reducing the potential for product loss. Pall filter plates allow you to remove, wash or label cells by vacuum or centrifugation simply, quickly, and easily.